Media recipes
Below are our suggested recipes for culturing media. You will find the recommended medium for each fungus type on their catalogue page.
Carnation Leaf Agar (CLA)
Add several sterile dried carnation leaf pieces, preferably gamma-irradiated, to Petri dishes just prior to addition of 10 to 15 mL of TWA.
Czapek Yeast Extract Agar (CYA)
Ingredients
| Ingredient | Amount |
|---|---|
| K2HPO4 | 1.0g |
| NaNO3 | 3.0 g |
| MgSO4.7H2O | 0.5 g |
| KCl | 0.5 g |
| FeSO4.7H2O | 0.01 g |
| Sucrose | 30.0 g |
| Yeast extract | 5.0 g |
| Agar | 15.0 g |
| Water, distilled | 1.0 L |
Method
- Add 1.0 mL/L of trace metals solution.
- Sterilise by autoclaving at 121°C for 15 minutes.
(Trace metals solution: 1% w/v ZnSO4.7H2O and 0.5% w/v CuSO4.5H2O in distilled water).
Czapek Yeast Extract Agar with 20% Sucrose (CY20S)
Ingredients
| Ingredient | Amount |
|---|---|
| K2HPO4 | 1.0 g |
| NaNO3 | 3.0 g |
| MgSO4.7H2O | 0.5 g |
| KCl | 0.5 g |
| FeSO4.7H2O | 0.01 g |
| Sucrose | 200.0 g |
| Yeast extract | 5.0 g |
| Agar | 15.0 g |
| Water, distilled | 1.0 L |
Method
Sterilise by autoclaving at 121°C for 15 minutes.
Dichloran Chloramphenicol Malt Agar (DCMA)
Ingredients
| Ingredient | Amount |
|---|---|
| Malt extract | 10 g |
| Dichloran (0.2% w/v in 95% ethanol) | .0 mL |
| Chloramphenicol | 0.1 g |
| Agar | 15.0 g |
| Water, distilled | to 1.0 L |
Method
Sterilise by autoclaving at 121°C for 15 minutes.
Malt Extract Agar (MEA)
Ingredients
| Ingredient | Amount |
|---|---|
| Malt extract (powdered) | 20.0 g |
| Glucose | 20.0 g |
| Peptone | 1.0 g |
| Agar | 20.0 g |
| Water, distilled | 1.0 L |
Method
Sterilise by autoclaving at 121°C for 15 minutes.
Malt Extract Yeast Extract 50% Glucose Agar (MY50G)
Ingredients
| Ingredient | Amount |
|---|---|
| Malt extract (powdered) | 10.0 g |
| Yeast extract | 2.5 g |
| Agar | 10.0 g |
| Water, distilled | 500.0 g |
| Glucose A.R. | 500.0 g |
Method
- Add minor constituents and agar to 500.0 g distilled water and steam to dissolve agar.
- Add glucose rapidly while stirring.
- Sterilise by steaming for 30 minutes. Do not autoclave.
Malt Extract Yeast Extract 5% Salt 12% Glucose Agar (MY5-12)
Ingredients
| Ingredient | Amount |
|---|---|
| Malt extract (powdered) | 20.0 g |
| Yeast extract | 5.0 g |
| NaCl | 50.0 g |
| Glucose | 120.0 g |
| Agar | 20.0 g |
| Water, distilled | to 1.0 L |
Method
Sterilise by autoclaving at 121°C for 10 minutes.
Potato Dextrose Agar (PDA)
Ingredients
| Ingredient | Amount |
|---|---|
| Potatoes | 250.0 g |
| Glucose | 20.0 g |
| Agar | 15.0 g |
| Water, distilled | 1.0 L |
Method
- Dice unpeeled potatoes into 500 mL of water
- Steam or boil for 30 to 45 minutes.
- Melt agar in 500 mL of water.
- Strain potato through several layers of cheese cloth into a flask containing the melted agar.
- Squeeze some potato pulp through the cheese cloth as well.
- Add the glucose and mix thoroughly, make up to 1 L with distilled water.
- Sterilise by autoclaving at 121°C for 15 minutes.
Tap water Agar (TWA)
Ingredients
| Ingredient | Amount |
|---|---|
| Agar | 20.0 g |
| Water, distilled | 1.0 L |
Method
Sterilise by autoclaving at 121°C for 15 minutes.