Media recipes

Below are our suggested recipes for culturing media. You will find the recommended medium for each fungus type on their catalogue page.

Carnation Leaf Agar (CLA)

Add several sterile dried carnation leaf pieces, preferably gamma-irradiated, to Petri dishes just prior to addition of 10 to 15 mL of TWA.

Czapek Yeast Extract Agar (CYA)

Ingredients

IngredientAmount
K2HPO4 1.0g
NaNO3 3.0 g
MgSO4.7H2O 0.5 g
KCl 0.5 g
FeSO4.7H2O 0.01 g
Sucrose 30.0 g
Yeast extract 5.0 g
Agar 15.0 g
Water, distilled 1.0 L

Method

  1. Add 1.0 mL/L of trace metals solution.
  2. Sterilise by autoclaving at 121°C for 15 minutes.

(Trace metals solution: 1% w/v ZnSO4.7H2O and 0.5% w/v CuSO4.5H2O in distilled water).

Czapek Yeast Extract Agar with 20% Sucrose (CY20S)

Ingredients

IngredientAmount
K2HPO4 1.0 g
NaNO3 3.0 g
MgSO4.7H2O 0.5 g
KCl 0.5 g
FeSO4.7H2O 0.01 g
Sucrose 200.0 g
Yeast extract 5.0 g
Agar 15.0 g
Water, distilled 1.0 L

Method

Sterilise by autoclaving at 121°C for 15 minutes.

Dichloran Chloramphenicol Malt Agar (DCMA)

Ingredients

IngredientAmount
Malt extract 10 g
Dichloran (0.2% w/v in 95% ethanol) .0 mL
Chloramphenicol 0.1 g
Agar 15.0 g
Water, distilled to 1.0 L

Method

Sterilise by autoclaving at 121°C for 15 minutes.

Malt Extract Agar (MEA)

Ingredients

IngredientAmount
Malt extract (powdered) 20.0 g
Glucose 20.0 g
Peptone 1.0 g
Agar 20.0 g
Water, distilled 1.0 L

Method

Sterilise by autoclaving at 121°C for 15 minutes.

Malt Extract Yeast Extract 50% Glucose Agar (MY50G)

Ingredients

IngredientAmount
Malt extract (powdered) 10.0 g
Yeast extract 2.5 g
Agar 10.0 g
Water, distilled 500.0 g
Glucose A.R. 500.0 g

Method

  1. Add minor constituents and agar to 500.0 g distilled water and steam to dissolve agar.
  2. Add glucose rapidly while stirring.
  3. Sterilise by steaming for 30 minutes. Do not autoclave.

Malt Extract Yeast Extract 5% Salt 12% Glucose Agar (MY5-12)

Ingredients

IngredientAmount
Malt extract (powdered) 20.0 g
Yeast extract 5.0 g
NaCl 50.0 g
Glucose 120.0 g
Agar 20.0 g
Water, distilled to 1.0 L

Method

Sterilise by autoclaving at 121°C for 10 minutes.

Potato Dextrose Agar (PDA)

Ingredients

IngredientAmount
Potatoes 250.0 g
Glucose 20.0 g
Agar 15.0 g
Water, distilled 1.0 L

Method

  1. Dice unpeeled potatoes into 500 mL of water
  2. Steam or boil for 30 to 45 minutes.
  3. Melt agar in 500 mL of water.
  4. Strain potato through several layers of cheese cloth into a flask containing the melted agar.
  5. Squeeze some potato pulp through the cheese cloth as well.
  6. Add the glucose and mix thoroughly, make up to 1 L with distilled water.
  7. Sterilise by autoclaving at 121°C for 15 minutes.

Tap water Agar (TWA)

Ingredients

IngredientAmount
Agar 20.0 g
Water, distilled 1.0 L

Method

Sterilise by autoclaving at 121°C for 15 minutes.